Time-domain fluorescence lifetime imaging applied to biological tissue

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Time-domain fluorescence lifetime imaging applied to biological tissue.

Fluorescence lifetime imaging (FLIM) is a functional imaging methodology that can provide information, not only concerning the localisation of specific fluorophores, but also about the local fluorophore environment. It may be implemented in scanning confocal or multi-photon microscopes, or in wide-field microscopes and endoscopes. When applied to tissue autofluorescence, it reveals intrinsic ex...

متن کامل

Enhancing precision in time-domain fluorescence lifetime imaging.

In biological applications of fluorescence lifetime imaging, low signals from samples can be a challenge, causing poor lifetime precision. We demonstrate how optimal signal gating (a method applied to the temporal dimension of a lifetime image) and novel total variation denoising models (a method applied to the spatial dimension of a lifetime image) can be used in time-domain fluorescence lifet...

متن کامل

Time-domain whole-field fluorescence lifetime imaging with optical sectioning.

A whole-field time-domain fluorescence lifetime imaging (FLIM) microscope with the capability to perform optical sectioning is described. The excitation source is a mode-locked Ti:Sapphire laser that is regeneratively amplified and frequency doubled to 415 nm. Time-gated fluorescence intensity images at increasing delays after excitation are acquired using a gated microchannel plate image inten...

متن کامل

GPU acceleration of time-domain fluorescence lifetime imaging.

Fluorescence lifetime imaging microscopy (FLIM) plays a significant role in biological sciences, chemistry, and medical research. We propose a graphic processing unit (GPU) based FLIM analysis tool suitable for high-speed, flexible time-domain FLIM applications. With a large number of parallel processors, GPUs can significantly speed up lifetime calculations compared to CPU-OpenMP (parallel com...

متن کامل

Fluorescence lifetime measurements and biological imaging.

6.4.1. Transition Metal Luminescent Complexes 2669 6.4.2. Metalloporphyrins 2669 6.4.3. Lanthanide Complexes 2670 6.4.4. Upconversion 2671 7. Fluorescence Lifetime Imaging Applications 2671 7.1. Cell Biology 2672 7.2. Tissue and Small Animal Lifetime Imaging 2672 7.2.1. Imaging of Cancer 2672 7.2.2. Diabetes and Kidney Pathologies 2674 7.2.3. Brain Tissue Analysis 2674 7.2.4. Blood Pathologies ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Photochemical & Photobiological Sciences

سال: 2004

ISSN: 1474-905X,1474-9092

DOI: 10.1039/b316456j